1N6J

Structural basis of sequence-specific recruitment of histone deacetylases by Myocyte Enhancer Factor-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 14-BM-C
Synchrotron site	APS
Beamline	14-BM-C
Temperature [K]	101
Detector technology	CCD
Detector	ADSC QUANTUM 4
Spacegroup name	P 41 2 2
Unit cell lengths	70.140, 70.140, 151.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.243
Rwork	0.243
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1egw
RMSD bond length	0.007 *
RMSD bond angle	1.090 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.063 *	0.319 *
Total number of observations	364592 *
Number of reflections	18733
<I/σ(I)>	38	4.4
Completeness [%]	92.7 *	77.7 *
Redundancy	19.5	4.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.2	17 *	PEG 3500, sodium cholride, BTP, pH 7.2, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents

ID	crystal ID	solution ID	reagent name	concentration	details
1	1	1	PEG 3500
2	1	1	sodium cholride
3	1	1	BTP
4	1	1	H2O
5	1	2	PEG 3500
6	1	2	sodium cholride
7	1	2	H2O

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	bis-Tris-propane	50 (mM)	pH6.35-6.68
2	1	reservoir	PEG1000	15 (%)
3	1	reservoir		50 (mM)
4	1	reservoir		10 (mM)
5	1	reservoir		5 (mM)
6	1	reservoir	glycerol	10 (%)
7	1	reservoir	dithiothreitol	5 (mM)
8	1	reservoir	spermine	2 (mM)