1N6A
Structure of SET7/9
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 103 |
| Wavelength(s) | 0.9792, 0.9791, 0.9464 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 102.231, 38.668, 66.596 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 1.700 |
| R-factor | 0.2 |
| Rwork | 0.194 |
| R-free | 0.21700 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.250 * |
| Data reduction software | DENZO |
| Phasing software | SOLVE |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 100.000 * |
| High resolution limit [Å] | 1.700 |
| Rmerge | 0.054 * |
| Total number of observations | 300831 * |
| Number of reflections | 29855 * |
| Completeness [%] | 99.7 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 4 * | PEG6000, Tris-HCl, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 50 (mM) | pH7.5 |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 5 (mM) | |
| 5 | 1 | drop | AdoMet | 5 (mM) | |
| 6 | 1 | reservoir | Tris | 100 (mM) | pH8.5 |
| 7 | 1 | reservoir | PEG6000 | 30 (%) |
