1N6A

Structure of SET7/9

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	103
Wavelength(s)	0.9792, 0.9791, 0.9464
Spacegroup name	P 21 21 2
Unit cell lengths	102.231, 38.668, 66.596
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 1.700
R-factor	0.2
Rwork	0.194
R-free	0.21700
Structure solution method	MAD
RMSD bond length	0.005
RMSD bond angle	1.250 *
Data reduction software	DENZO
Phasing software	SOLVE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall
Low resolution limit [Å]	100.000 *
High resolution limit [Å]	1.700
Rmerge	0.054 *
Total number of observations	300831 *
Number of reflections	29855 *
Completeness [%]	99.7 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	4 *	PEG6000, Tris-HCl, DTT, pH 8.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20 (mg/ml)
2	1	drop	Tris-HCl	50 (mM)	pH7.5
3	1	drop		200 (mM)
4	1	drop	dithiothreitol	5 (mM)
5	1	drop	AdoMet	5 (mM)
6	1	reservoir	Tris	100 (mM)	pH8.5
7	1	reservoir	PEG6000	30 (%)