1N5U

X-RAY STUDY OF HUMAN SERUM ALBUMIN COMPLEXED WITH HEME

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2001-08-08
Detector	ADSC QUANTUM 4
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	183.116, 37.909, 94.832
Unit cell angles	90.00, 105.04, 90.00

Refinement procedure

Resolution	50.000 * - 1.900
Rwork	0.235
R-free	0.28200 *
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.190 *
Phasing software	MERLOT
Refinement software	CNX (2000.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.037 *	0.266 *
Number of reflections	45420
Completeness [%]	83.5 *	56.3 *
Redundancy	2.6 *	2.11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.5	293	Carter, D.C., (1994) Eur. J. Biochem., 226, 1049. *