1N5D

CRYSTAL STRUCTURE OF PORCINE TESTICULAR CARBONYL REDUCTASE/ 20BETA-HYDROXYSTEROID DEHYDROGENASE

Replaces:  1HU4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1992-11-10
Detector	RIGAKU RAXIS IIC
Spacegroup name	P 43 21 2
Unit cell lengths	58.530, 58.530, 165.640
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.200 * - 2.300
R-factor	0.194
Rwork	0.194
R-free	0.25100
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.620
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.200 *	2.500
High resolution limit [Å]	2.300	2.300
Rmerge	0.066
Number of reflections	10792
<I/σ(I)>	9.8
Completeness [%]	80.0	52
Redundancy	3.0 *	2.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	other *	6		Ghosh, D., (1993) J. Steroid Biochem. Mol. Biol., 46, 103. *