1N57
Crystal Structure of Chaperone Hsp31
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 200 |
| Detector technology | CCD |
| Collection date | 2002-08-16 |
| Detector | APS-1 |
| Wavelength(s) | 0.9793, 0.9794, 0.9641 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 52.150, 82.000, 64.480 |
| Unit cell angles | 90.00, 100.00, 90.00 |
Refinement procedure
| Resolution | 20.000 * - 1.600 |
| R-factor | 0.18979 |
| Rwork | 0.187 |
| R-free | 0.24200 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.301 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.1.24) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.670 |
| High resolution limit [Å] | 1.600 | 1.610 |
| Rmerge | 0.098 * | 0.418 * |
| Number of reflections | 35209 | |
| <I/σ(I)> | 12.8 | 1.96 |
| Completeness [%] | 97.8 * | 88.7 * |
| Redundancy | 5.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 14 * | PEG 3350,300 mM MgCl2 50mM Mes 4mM DTT, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 273K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | Tris | 50 (mM) | pH7.5 |
| 3 | 1 | drop | EDTA | 1 (mM) | |
| 4 | 1 | drop | dithiothreitol | 4 (mM) | |
| 5 | 1 | reservoir | PEG3350 | 25 (%) | |
| 6 | 1 | reservoir | 300 (mM) | ||
| 7 | 1 | reservoir | MES | 50 (mM) | pH6.5 |
| 8 | 1 | reservoir | dithiothreitol | 4 (mM) |
