1N4X

Structure of scFv 1696 at acidic pH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID2
Synchrotron site	ESRF
Beamline	ID2
Detector technology	AREA DETECTOR
Collection date	1998-01-06
Detector	MARRESEARCH
Wavelength(s)	0.99
Spacegroup name	P 21 21 21
Unit cell lengths	126.930, 61.210, 57.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.700
R-factor	0.227
Rwork	0.227
R-free	0.25600
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	1.340
Data reduction software	XDS
Data scaling software	XDS
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	1.700 *
Rmerge	0.090 *	0.360 *
Number of reflections	45422 *	5930 *
Completeness [%]	91.0 *	84.2 *
Redundancy	4.89 *	3.34 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.3 *	291 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	sodium citrate	0.1 (M)	pH3.5
2	1	reservoir	PEG4000	17.5 (%)
3	1	reservoir	ammonium sulfate	0.175 (M)
4	1	drop	protein	5 (mg/ml)
5	1	drop	Tris-HCl	10 (mM)	pH7.3