1N43

Streptavidin Mutant N23A with biotin at 1.89A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	120
Detector technology	IMAGE PLATE
Collection date	1996-09-19
Detector	RIGAKU RAXIS II
Wavelength(s)	1.5418
Spacegroup name	P 1 21 1
Unit cell lengths	52.180, 97.200, 51.580
Unit cell angles	90.00, 112.60, 90.00

Refinement procedure

Resolution	10.000 - 1.900 *
R-factor	0.1917
Rwork	0.192
R-free	0.30100 *
Structure solution method	isomorphous replacement
Starting model (for MR)	1swe
RMSD bond length	0.005
RMSD bond angle	0.021
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELX
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	1.900 *
Rmerge	0.070	0.250 *
Number of reflections	27213 *
Completeness [%]	71.0	43 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.5	293	MPD, pH 4.5, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	20-30 (mg/ml)
2	1	reservoir	MPD	50 (%)