1N42
Crystal Structure of Annexin V R149E Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU RAXIS IIC |
| Wavelength(s) | 1.5418 |
| Spacegroup name | H 3 |
| Unit cell lengths | 157.535, 157.535, 36.383 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 50.000 - 2.100 |
| R-factor | 0.1927 |
| Rwork | 0.193 |
| R-free | 0.23950 |
| Structure solution method | FOURIER SYNTHESIS |
| RMSD bond length | 1.112 |
| RMSD bond angle | 0.006 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.077 * | 0.292 * |
| Number of reflections | 22834 | |
| Completeness [%] | 99.7 * | 99.5 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8.2 | 290 | ammonium sulfate, CaCl2, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 290K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 34-40 (%sat) | |
| 2 | 1 | reservoir | HEPES | 50 (mM) | pH8.2 |
| 3 | 1 | reservoir | dithiothreitol | 2 (mM) | |
| 4 | 1 | reservoir | 10 (mM) | ||
| 5 | 1 | drop | protein | 14 (mg/ml) | |
| 6 | 1 | drop | 20 (mM) |
