1N3X

Ligand-free High-Affinity Maltose-Binding Protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2001-09-30
Detector	MARRESEARCH
Wavelength(s)	1.5479
Spacegroup name	P 1 21 1
Unit cell lengths	44.590, 71.330, 59.010
Unit cell angles	90.00, 101.53, 90.00

Refinement procedure

Resolution	23.800 * - 2.500
R-factor	0.197
Rwork	0.197
R-free	0.23400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1omp
RMSD bond length	0.007
RMSD bond angle	1.000 *
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.790	2.590
High resolution limit [Å]	2.500	2.480
Rmerge	0.083	0.174
Number of reflections	12399 *
<I/σ(I)>	21	9.5
Completeness [%]	98.0 *	97.8
Redundancy	7.6 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	290	PEG 4000, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	cacodylate	10 (mM)	pH6.5
2	1	reservoir		200 (mM)
3	1	reservoir	zinc acetate	18 (mM)
4	1	reservoir	PEG4000	24 (%)
5	1	reservoir	maltose	1 (mM)