1N3U
Crystal structure of human heme oxygenase 1 (HO-1) in complex with its substrate heme, crystal form B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-05-05 |
| Detector | RIGAKU RAXIS IV |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.395, 55.920, 79.379 |
| Unit cell angles | 90.00, 101.20, 90.00 |
Refinement procedure
| Resolution | 70.000 - 2.600 * |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QQ8 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.200 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.000 | 2.640 |
| High resolution limit [Å] | 2.600 * | 2.600 * |
| Rmerge | 0.074 * | 0.566 * |
| Total number of observations | 100842 * | |
| Number of reflections | 14273 * | |
| <I/σ(I)> | 10.1 | 3 |
| Completeness [%] | 96.9 | 94.4 |
| Redundancy | 7.1 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 * | 28 * | ammonium sulfate, HEPES, 1,6-hexanediol, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 301K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | ammonium sulfate | 2.08 (M) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 3 | 1 | reservoir | 1,6-hexanediol | 0.9 (%) | |
| 4 | 1 | drop | protein | 35 (mg/ml) | |
| 5 | 1 | drop | potassium phosphate | 20 (mM) | pH7.4 |
