1N3R

Biosynthesis of pteridins. Reaction mechanism of GTP cyclohydrolase I

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-10
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	224.466, 313.258, 130.181
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.221
Rwork	0.218
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.510
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	CNS

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.800
Number of reflections	105682
Completeness [%]	97.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.2	293	Peg 6000, pH 8.2, VAPOR DIFFUSION, SITTING DROP, temperature 293K