1N3O
Pterocarcpus angolensis lectin in complex with alpha-methyl glucose
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-09-24 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.84 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.760, 83.550, 122.930 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.090 | 0.311 |
| Total number of observations | 173945 * | |
| Number of reflections | 39215 | 3190 * |
| <I/σ(I)> | 12.43 | |
| Completeness [%] | 97.4 | 80.5 |
| Redundancy | 4.44 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.45 * | 300 | Ca-acetate, PEG-8000, Na-cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 300K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | sugar | 10 (mM) | |
| 3 | 1 | drop | Tris-HCl | 20 (mM) | pH7.45 |
| 4 | 1 | reservoir | calcium acetate | 200 (mM) | |
| 5 | 1 | reservoir | sodium cacodylate | 100 (mM) | pH6.5 |
| 6 | 1 | reservoir | PEG8000 | 20 (%(w/v)) |
