1N1Q

Crystal structure of a Dps protein from Bacillus brevis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1999-08-01
Detector	MARRESEARCH
Wavelength(s)	1.5418
Spacegroup name	H 3
Unit cell lengths	86.911, 86.911, 220.808
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.530 - 2.200
R-factor	0.1958
Rwork	0.196
R-free	0.20800 *
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.200 *
Data scaling software	SCALEPACK
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.530	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.085	0.196
Total number of observations	179534 *
Number of reflections	31582
<I/σ(I)>	21.3	8.1
Completeness [%]	99.7	98.3
Redundancy	15.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	293	phosphate, pH 7.4, VAPOR DIFFUSION, SITTING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	Tris-HCl	10 (mM)	pH7.4
2	1	reservoir	sodium potassium phosphate	1.6 (M)
3	1	reservoir	sodium HEPES	0.1 (M)	pH7.4