1N1H
Initiation complex of polymerase lambda3 from reovirus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-11-01 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 1.03 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 70.883, 85.013, 249.294 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26100 * |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | lambda3 native structure |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.395 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.120 | 0.283 |
| Number of reflections | 30558 | |
| <I/σ(I)> | 15 | 2.3 |
| Completeness [%] | 79.4 | 75.6 |
| Redundancy | 2-3 * | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 | 22 * | PEG4000, sodium chloride, HEPES, glycerol, pH 7.8, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | PEG 4000 | ||
| 2 | 1 | 1 | NaCl | ||
| 3 | 1 | 1 | HEPES | ||
| 4 | 1 | 1 | glycerol | ||
| 5 | 1 | 2 | PEG 4000 | ||
| 6 | 1 | 2 | NaCl | ||
| 7 | 1 | 2 | glycerol | ||
| 8 | 1 | 2 | HEPES |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8.7 (mg/ml) | |
| 2 | 1 | drop | sodium HEPES | 50 (mM) | pH7.8 |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | 150 (mM) | ||
| 5 | 1 | drop | dithiothreitol | 10 (mM) | |
| 6 | 1 | drop | 0.02 (%) | ||
| 7 | 1 | reservoir | PEG8000 | 7.5 (%) | |
| 8 | 1 | reservoir | glycerol | 10 (%) | |
| 9 | 1 | reservoir | sodium HEPES | 0.1 (M) | pH7.8 |
