1N1F

Crystal Structure of Human Interleukin-19

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2002-03-22
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	30.657, 52.974, 93.474
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.950
R-factor	0.156
Rwork	0.156
R-free	0.24300
Structure solution method	MIR
RMSD bond length	0.018 *
RMSD bond angle	21.800 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.020
High resolution limit [Å]	1.950	1.950
Rmerge	0.065	0.268
Number of reflections	10032 *
<I/σ(I)>	15.8	2.6
Completeness [%]	83.6 *	62.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.9	21 *	1.9M Ammonium sulfate, 2% PEG400, 100mM HEPES, pH 6.9, VAPOR DIFFUSION, HANGING DROP, temperature 273K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	HEPES	100 (mM)
2	1	reservoir	ammonium sulfate	1.9 (M)
3	1	reservoir	PEG400	2 (%)	pH6.9
4	1	drop	protein	7 (mg/ml)