1N1C
Crystal Structure Of The Dimeric TorD Chaperone From Shewanella Massilia
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7A |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-08-21 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.97856, 0.95370, 0.97872 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 65.958, 93.441, 95.136 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.400 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.25500 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 19.700 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 54.230 * | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.033 | 0.177 |
| Total number of observations | 85570 * | |
| Number of reflections | 23239 | |
| <I/σ(I)> | 14 | 3.6 |
| Completeness [%] | 98.7 | 98.9 |
| Redundancy | 3.7 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 4 * | ammonium sulfate 1.6M, MES 100mM; Crystals were obtained by mixing 1 microl (1.2mg.ml-1) of protein solution with an equal volume of reservoir solution; Crystal Size (300x200x100 microM3), pH 6.4, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 1.2 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | pH8.0 |
| 3 | 1 | drop | 240 (mM) | ||
| 4 | 1 | drop | dithiothreitol | 10 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.7 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) | pH6.6 |
