1N0U
Crystal structure of yeast elongation factor 2 in complex with sordarin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-04-02 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.811 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 119.960, 150.830, 65.070 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.120 |
| R-factor | 0.239 |
| Rwork | 0.236 |
| R-free | 0.25300 * |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.300 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.230 |
| High resolution limit [Å] | 2.120 | 2.120 |
| Rmerge | 0.062 * | 0.340 * |
| Number of reflections | 66427 | |
| <I/σ(I)> | 34.3 | 1.7 |
| Completeness [%] | 98.3 * | 94 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 7.2 | 277 * | Joergensen, R., (2002) Acta Crystallogr., Sect.D, 58, 712. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES-NaOH | 20 (mM) | pH7.2 |
| 10 | 1 | reservoir | dithiothreitol | 3 (mM) | |
| 11 | 1 | reservoir | 0.9 (mM) | ||
| 12 | 1 | reservoir | PEG8000 | 7-9 (%) | |
| 2 | 1 | drop | 75 (mM) | ||
| 3 | 1 | drop | 5 (mM) | ||
| 4 | 1 | drop | EDTA | 0.5 (mM) | |
| 5 | 1 | drop | dithiothreitol | 0.5 (mM) | |
| 6 | 1 | drop | GDP | 1 (mM) | |
| 7 | 1 | drop | protein | 4.5 (mg/ml) | |
| 8 | 1 | reservoir | HEPES | 0.1 (M) | pH7.2 |
| 9 | 1 | reservoir | ethylene glycol | 5 (%) |
