1N0J

The Structure of Human Mitochondrial MN3+ Superoxide Dismutase Reveals a Novel Tetrameric Interface of Two 4-Helix Bundles

Replaces:  1ABM

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	298
Detector technology	AREA DETECTOR
Detector	SIEMENS
Wavelength(s)	1.5418
Spacegroup name	P 21 21 2
Unit cell lengths	76.130, 79.900, 68.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.200
R-factor	0.171
Rwork	0.171
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.018
RMSD bond angle	3.300
Data reduction software	X-GEN
Refinement software	X-PLOR (20)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.300
High resolution limit [Å]	2.200	2.200
Rmerge	0.090
Total number of observations	117362 *
Number of reflections	19738
Completeness [%]	92.0	52
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8 *	293	peg 600, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	phosphate	50 (mM)	pH7.8
3	1	reservoir	PEG400	24-30 (%)