1N04
Diferric chicken serum transferrin at 2.8 A resolution.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2002-05-17 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 72.957, 59.112, 81.860 |
| Unit cell angles | 90.00, 95.56, 90.00 |
Refinement procedure
| Resolution | 15.000 * - 2.800 |
| R-factor | 0.216 |
| Rwork | 0.216 |
| R-free | 0.26900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Diferric chicken ovotransferrin at 2.4 A resolution |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.370 * |
| Total number of observations | 155917 * | |
| Number of reflections | 17301 * | |
| <I/σ(I)> | 9.71 | 2.12 |
| Completeness [%] | 92.3 * | 92.4 * |
| Redundancy | 9.01 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.9 * | 277 | PEG6000, 0.02M acetate, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 18-21 (%) | |
| 2 | 1 | reservoir | sodium cacodylate | 40 (mM) | pH5.9 |
| 3 | 1 | reservoir | 20 (mM) | ||
| 4 | 1 | drop | protein | 40 (mg/ml) | |
| 5 | 1 | drop | sodium cacodylate | 40 (mM) | pH5.9 |
