1MZO
Crystal structure of pyruvate formate-lyase with pyruvate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-06-20 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9102 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 158.355, 158.355, 159.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.700 |
| Rwork | 0.196 |
| R-free | 0.23500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2pfl |
| RMSD bond length | 0.007 * |
| RMSD bond angle | 1.200 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.800 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.111 * | 0.340 * |
| Total number of observations | 318766 * | |
| Number of reflections | 55923 | |
| Completeness [%] | 98.8 | 94.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7.3 | 298 | PEG1000, MOPS, ammonium chloride, na-pyruvate, DTT, magnesium chloride, EDTA, pH 7.3, MICRODIALYSIS, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | MOPS/NH3 | 50 (mM) | |
| 10 | 1 | 2 | 2 (mM) | ||
| 2 | 1 | 1 | dithiothreitol | 20 (mM) | |
| 3 | 1 | 1 | protein | 25 (mg/ml) | |
| 4 | 1 | 2 | MOPS/NH3 | 25 (mM) | pH7.3 |
| 5 | 1 | 2 | PEG1000 | 25 (%(w/v)) | |
| 6 | 1 | 2 | dithiothreitol | 10 (mM) | |
| 7 | 1 | 2 | 25 (mM) | ||
| 8 | 1 | 2 | sodium pyruvate | 25 (mM) | |
| 9 | 1 | 2 | EDTA | 1 (mM) |
