1MZG

X-Ray Structure of SufE from E.coli Northeast Structural Genomics (NESG) Consortium Target ER30

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Collection date	2002-08-23
Spacegroup name	P 21 21 21
Unit cell lengths	42.190, 54.401, 120.685
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.900 * - 2.000
Rwork	0.208
R-free	0.25100
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	21.500 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.900 *
High resolution limit [Å]	2.000
Rmerge	0.054 *
Number of reflections	17893
Completeness [%]	98.6 *	89.3 *
Redundancy	3.1 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.9	30 *	34% PEG3350, 3% Methanol, 0.1M Tris pH 8.9, 0.2M Sodium acetate, VAPOR DIFFUSION, HANGING DROP, temperature 302.0K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350	34 (%(w/v))
2	1	reservoir	sodium acetate	200 (mM)
3	1	reservoir	methanol	3 (%(v/v))
4	1	reservoir	Tris-HCl	100 (mM)	pH8.9