1MZE
Human Factor Inhibiting HIF (FIH1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2002-07-29 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.97916 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 86.620, 86.620, 147.760 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 * - 2.200 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.23700 |
| Structure solution method | SAD |
| Starting model (for MR) | SeMet FIH-1 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.450 * |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 * | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.062 * | 0.484 * |
| Total number of observations | 322469 * | |
| Number of reflections | 29190 * | |
| <I/σ(I)> | 35.7 | 4.9 |
| Completeness [%] | 99.9 * | 100 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 * | 21 * | potassium tartrate, CAPSO, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 5 (mM) | pH8.0 |
| 3 | 1 | drop | 50 (mM) | ||
| 4 | 1 | drop | 2-mercaptoethanol | 5 (mM) | |
| 5 | 1 | reservoir | CAPSO | 50 (mM) | pH9.6 |
| 6 | 1 | reservoir | potassium tartrate | 0.9-1.2 (M) | |
| 7 | 1 | reservoir | glycerol | 30 (%) |
