1MZE

Human Factor Inhibiting HIF (FIH1)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-29
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97916
Spacegroup name	P 41 21 2
Unit cell lengths	86.620, 86.620, 147.760
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.200
R-factor	0.218
Rwork	0.216
R-free	0.23700
Structure solution method	SAD
Starting model (for MR)	SeMet FIH-1
RMSD bond length	0.010
RMSD bond angle	1.450 *
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000 *	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.062 *	0.484 *
Total number of observations	322469 *
Number of reflections	29190 *
<I/σ(I)>	35.7	4.9
Completeness [%]	99.9 *	100 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8 *	21 *	potassium tartrate, CAPSO, glycerol, pH 9.6, VAPOR DIFFUSION, HANGING DROP, temperature 294K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH8.0
3	1	drop		50 (mM)
4	1	drop	2-mercaptoethanol	5 (mM)
5	1	reservoir	CAPSO	50 (mM)	pH9.6
6	1	reservoir	potassium tartrate	0.9-1.2 (M)
7	1	reservoir	glycerol	30 (%)