1MZ5

Trypanosoma rangeli sialidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-07
Detector	MARRESEARCH
Wavelength(s)	0.95
Spacegroup name	P 21 21 21
Unit cell lengths	76.150, 93.410, 105.480
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 2.200
R-factor	0.18327
Rwork	0.180
R-free	0.26300 *
Structure solution method	MIR + Molecular Replacement
RMSD bond length	0.018 *
RMSD bond angle	0.045 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE (+ MLPHARE)
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.126 *	0.354 *
Total number of observations	210923 *
Number of reflections	38612
Completeness [%]	99.3	97.7
Redundancy	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	18 *	PEG-8000, ammonium sulfate, morpholinoethanesulfonate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	enzyme	7 (mg/ml)
2	1	reservoir	PEG8000	17 (%)
3	1	reservoir	ammonium sulfate	100 (mM)
4	1	reservoir	MOPS	50 (mM)	pH6.5