1MZ0

STRUCTURE OF MYOGLOBIN MB-YQR 316 ns AFTER PHOTOLYSIS OF CARBON MONOXIDE SOLVED FROM LAUE DATA AT RT.

Experimental procedure

Experimental method	LAUE
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID09
Synchrotron site	ESRF
Beamline	ID09
Temperature [K]	283
Detector technology	CCD
Collection date	2001-12-18
Detector	MAR CCD 130 mm
Spacegroup name	P 6
Unit cell lengths	91.200, 91.200, 45.712
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	13.000 - 1.600
R-factor	0.15
Rwork	0.150
R-free	0.17400 *
Structure solution method	Rigid body from starting model.
Starting model (for MR)	pdb id 1MYZ
RMSD bond length	0.009 *
RMSD bond angle	1.430 *
Data reduction software	PROW (+ CCP4)
Data scaling software	PROW
Phasing software	CNS (1.0)
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	1.620
High resolution limit [Å]	1.550	1.550
Rmerge	0.059 *	0.082 *
Total number of observations	79853 *
Number of reflections	25942
<I/σ(I)>	13	3.3
Completeness [%]	80.1	0.377 *
Redundancy	3.1	1.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	9 *	294	Phillips, G.N., (1990) Proteins Struct. Funct. Genet., 7, 358. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	70 (mg/ml)
2	1	1	ammonium sulfate	2.2-2.6 (M)
3	1	1	Tris-HCl	20 (mM)