1MYP

X-RAY CRYSTAL STRUCTURE OF CANINE MYELOPEROXIDASE AT 3 ANGSTROMS RESOLUTION

Experimental procedure

Spacegroup name	P 41 21 2
Unit cell lengths	133.000, 133.000, 203.600
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 * - 3.000
R-factor	0.257
RMSD bond length	0.011
RMSD bond angle	3.800
Refinement software	TNT

Data quality characteristics

	Overall
High resolution limit [Å]	3.100 *
Rmerge	0.077 *
Total number of observations	214889 *
Number of reflections	33190 *
Completeness [%]	97.0 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *		15-20 *	seeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	50 (mg/ml)
2	1	drop	ammonium phosphate	1.3 (M)
3	1	drop	PIPES	50 (mM)
4	1	reservoir	ammonium sulfate	1.7-1.8 (M)
5	1	reservoir	ammonium phosphate	1.3 (M)