1MYI

HIGH RESOLUTION X-RAY STRUCTURES OF PIG METMYOGLOBIN AND TWO CD3 MUTANTS MB(LYS45-> ARG) AND MB(LYS45-> SER)

Experimental procedure

Spacegroup name	I 1 21 1
Unit cell lengths	125.920, 42.920, 92.950
Unit cell angles	90.00, 92.24, 90.00

Refinement procedure

Resolution	10.000 - 2.000
R-factor	0.194
RMSD bond length	0.031
RMSD bond angle	0.079
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.000 *
Rmerge	0.052 *
Total number of observations	82597 *
Number of reflections	32815 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1 *	15 *	taken from Dodson, G. et al(1975). Nature, 257, 758. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	8 (mg/ml)
2	1	reservoir	sodium phosphate	50 (mM)
3	1	reservoir	ammonium sulfate	60-90 (%)