1MY7
NF-kappaB p65 subunit dimerization domain homodimer N202R mutation
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2001-11-25 |
Detector | SBC-2 |
Wavelength(s) | 1.000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 55.988, 75.072, 108.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 * - 1.490 |
R-factor | 0.247 |
Rwork | 0.233 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb id 1bft |
RMSD bond length | 0.005 |
RMSD bond angle | 1.430 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | AMoRE |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.540 |
High resolution limit [Å] | 1.490 | 1.490 |
Rmerge | 0.054 * | 0.327 * |
Total number of observations | 368554 * | |
Number of reflections | 36240 | |
<I/σ(I)> | 13.9 | 2.5 |
Completeness [%] | 91.8 | 82.7 |
Redundancy | 10.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.5 | 18 * | 0.l M sodium HEPES, 0.2 M sodium tartrate, 2 M ammonium sulfate, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 15 (mg/ml) | |
2 | 1 | reservoir | HEPES | 0.1 (M) | pH7.5 |
3 | 1 | reservoir | sodium tartrate | 0.15-0.2 (M) | |
4 | 1 | reservoir | ammonium sulfate | 1.9-2.1 (M) |