1MXU
CRYSTAL STRUCTURE OF THE GLUR2 LIGAND BINDING CORE (S1S2J) in complex with bromo-willardiine (Control for the crystal titration experiments)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2000-09-15 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.91843 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 114.292, 163.403, 48.072 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.370 - 1.800 |
R-factor | 0.211 |
Rwork | 0.211 |
R-free | 0.23400 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.005 |
RMSD bond angle | 21.700 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.061 | 0.203 |
Number of reflections | 76525 | |
Completeness [%] | 90.2 | 55.2 |
Redundancy | 4.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 4 * | PEG8K, ammonium sulfate, zinc acetate, BrW and sodium cacodylate, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | HEPES | 10 (mM) | pH7.0 |
2 | 1 | drop | 20 (mM) | ||
3 | 1 | drop | EDTA | 1 (mM) | |
4 | 1 | drop | protein | 10 (mg/ml) | |
5 | 1 | reservoir | PEG8000 | 12-16 (%) | |
6 | 1 | reservoir | ammonium sulfate | 0.25-0.3 (M) | |
7 | 1 | reservoir | zinc acetate | 0.05 (M) | |
8 | 1 | reservoir | sodium cacodylate | 0.1 (M) | pH6.5 |