1MWW

THE STRUCTURE OF THE HYPOTHETICAL PROTEIN HI1388.1 FROM HAEMOPHILUS INFLUENZAE REVEALS A TAUTOMERASE/MIF FOLD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-07
Detector	ADSC QUANTUM 210
Wavelength(s)	0.97240
Spacegroup name	I 41 2 2
Unit cell lengths	115.150, 115.150, 124.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.830 - 2.080
Rwork	0.198
R-free	0.26900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1otf
RMSD bond length	0.021
RMSD bond angle	1.900
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	EPMR
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.830	2.150
High resolution limit [Å]	2.080	2.080
Rmerge	0.048	0.305
Number of reflections	24983
<I/σ(I)>	15.5	8.96
Completeness [%]	98.3	89.6
Redundancy	14.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	294	50% MPD, 0.1M NACACODYLATE, 0.2M NAGLUTAMATE, pH 5.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K