1MWD

WILD TYPE DEOXY MYOGLOBIN

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	150
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS
Spacegroup name	I 1 21 1
Unit cell lengths	122.120, 42.140, 91.490
Unit cell angles	90.00, 92.85, 90.00

Refinement procedure

Resolution	24.000 - 1.800
R-factor	0.196 *
Rwork	0.196
R-free	0.24300
Structure solution method	OTHER
RMSD bond length	0.013
RMSD bond angle	0.030
Data reduction software	DENZO
Data scaling software	CCP4
Phasing software	CCP4
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000
High resolution limit [Å]	1.800
Rmerge	0.040 *	0.291 *
Number of reflections	42068
<I/σ(I)>	21.4
Completeness [%]	96.5
Redundancy	7.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.1		0.1 PHOSPHATE BUFFER 72-80% SATURATED AMMONIUM SULPHATE, pH 7.1

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	72-80 (%(v/v)sat)
2	1	drop	phosphate	0.1 (M)
3	1	drop	protein	10 (mg/ml)