1MW5
Structure of HI1480 from Haemophilus influenzae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2002-07-05 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.979 |
Spacegroup name | P 64 |
Unit cell lengths | 79.289, 79.289, 143.842 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.100 |
Rwork | 0.196 |
R-free | 0.24400 * |
Structure solution method | MAD |
RMSD bond length | 0.015 * |
RMSD bond angle | 1.600 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | SHELXD |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.200 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.055 * | 0.255 * |
Total number of observations | 341570 * | |
Number of reflections | 58739 * | |
<I/σ(I)> | 9 | 7.2 |
Completeness [%] | 99.7 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 8% PEG 8000, 0.2M MgCl2, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | reservoir | PEG8000 | 7 (%) | |
2 | 1 | reservoir | Tris-HCl | 0.1 (M) | pH7.0 |
3 | 1 | reservoir | 0.2 (M) | ||
4 | 1 | drop | protein | 20 (mg/ml) |