1MW5

Structure of HI1480 from Haemophilus influenzae

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2002-07-05
Detector	ADSC QUANTUM 4
Wavelength(s)	0.979
Spacegroup name	P 64
Unit cell lengths	79.289, 79.289, 143.842
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.100
Rwork	0.196
R-free	0.24400 *
Structure solution method	MAD
RMSD bond length	0.015 *
RMSD bond angle	1.600
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.200
High resolution limit [Å]	2.100	2.100
Rmerge	0.055 *	0.255 *
Total number of observations	341570 *
Number of reflections	58739 *
<I/σ(I)>	9	7.2
Completeness [%]	99.7	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	8% PEG 8000, 0.2M MgCl2, 0.1M Tris-HCl, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 298K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	7 (%)
2	1	reservoir	Tris-HCl	0.1 (M)	pH7.0
3	1	reservoir		0.2 (M)
4	1	drop	protein	20 (mg/ml)