1MVP

STRUCTURAL STUDIES OF THE RETROVIRAL PROTEINASE FROM AVIAN MYELOBLASTOSIS ASSOCIATED VIRUS

Experimental procedure

Spacegroup name	P 31 2 1
Unit cell lengths	88.920, 88.920, 78.790
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	5.000 - 2.200
R-factor	0.172
RMSD bond length	0.018
RMSD bond angle	0.034
Refinement software	PROLSQ

Data quality characteristics

	Overall
High resolution limit [Å]	2.200 *
Total number of observations	196542 *
Number of reflections	16968 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MAV proteinase	0.1 (mM)
2	1	drop	citrate	0.05 (M)	can be replaced with phosphate
3	1	drop	ammonium sulfate	8-12 (%sat)
4	1	drop	dimethyl formamide	5 (%(v/v))
5	1	drop	beta-mercaptoethanol	0.05 (%)
6	1	reservoir	citrate	0.05 (M)	can be replaced with phosphate
7	1	reservoir	ammonium sulfate	8-12 (%sat)
8	1	reservoir	dimethyl formamide	5 (%(v/v))
9	1	reservoir	beta-mercaptoethanol	0.05 (%)