1MVK
X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 95 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-10-07 |
| Detector | RIGAKU RAXIS II |
| Wavelength(s) | 1.54180 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 76.100, 210.400, 55.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.237 |
| Rwork | 0.237 |
| R-free | 0.28300 |
| Structure solution method | MAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.520 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASES |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.570 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.083 * | |
| Total number of observations | 182446 * | |
| Number of reflections | 31523 | |
| <I/σ(I)> | 11.1 | |
| Completeness [%] | 99.4 | 93.4 |
| Redundancy | 5.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 293 | PEG 8000, ammonium sulfate, sodium acetate, sodium chloride, TrisHCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 16 (mg/ml) | |
| 2 | 1 | reservoir | PEG8000 | 20 (%(w/v)) | |
| 3 | 1 | reservoir | ammonium sulfate | 150 (mM) | |
| 4 | 1 | reservoir | sodium acetate | 100 (mM) | pH5.6 |
