1MVK

X-ray structure of the tetrameric mutant of the B1 domain of streptococcal protein G

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	95
Detector technology	IMAGE PLATE
Collection date	2000-10-07
Detector	RIGAKU RAXIS II
Wavelength(s)	1.54180
Spacegroup name	P 21 21 2
Unit cell lengths	76.100, 210.400, 55.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 2.500
R-factor	0.237
Rwork	0.237
R-free	0.28300
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.520 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.570
High resolution limit [Å]	2.500	2.500
Rmerge	0.083 *
Total number of observations	182446 *
Number of reflections	31523
<I/σ(I)>	11.1
Completeness [%]	99.4	93.4
Redundancy	5.78

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	293	PEG 8000, ammonium sulfate, sodium acetate, sodium chloride, TrisHCl, pH 5.6, VAPOR DIFFUSION, HANGING DROP, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	16 (mg/ml)
2	1	reservoir	PEG8000	20 (%(w/v))
3	1	reservoir	ammonium sulfate	150 (mM)
4	1	reservoir	sodium acetate	100 (mM)	pH5.6