1MVA

STRUCTURE OF A PROTEIN CAPSID OF THE T45A MUTANT OF PHAGE MS2

Experimental procedure

Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	280
Detector technology	IMAGE PLATE
Collection date	1994-12
Detector	MARRESEARCH
Spacegroup name	H 3 2
Unit cell lengths	288.000, 288.000, 653.000
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 3.000
R-factor	0.194
Rwork	0.194
R-free	0.23700 *
Structure solution method	MOLECULAR
Starting model (for MR)	REFINED MODEL OF MS2 PROTEIN CAPSID
RMSD bond length	0.017
RMSD bond angle	27.000 *
Data reduction software	DENZO
Data scaling software	CCP4 ((AGROVATA)
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	3.200
High resolution limit [Å]	3.000	3.000
Rmerge	0.093	0.182
Number of reflections	179377	31098 *
Completeness [%]	89.0	85.2 *
Redundancy	1.7	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4 *	37 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	MS2	1.0 (%(w/v))
2	1	drop	sodium phosphate	0.2 (M)
3	1	drop	PEG6000	1.5 (%(w/v))
4	1	drop		0.02 (%(w/v))
5	1	reservoir	sodium phosphate	0.4 (M)