1MUY
CATALYTIC DOMAIN OF MUTY FROM ESCHERICHIA COLI
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 90 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 83.060, 48.950, 69.030 |
| Unit cell angles | 90.00, 123.37, 90.00 |
Refinement procedure
| Resolution | 20.000 - 1.400 |
| R-factor | 0.153 |
| R-free | 0.23100 |
| Structure solution method | MIRAS |
| RMSD bond length | 0.011 |
| RMSD bond angle | 0.027 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXL-97 |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.057 * | |
| Total number of observations | 95656 * | |
| Number of reflections | 42039 | |
| Completeness [%] | 92.0 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8.5 * | 15 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 400 (mM) | ||
| 3 | 1 | drop | glycerol | 20 (%) | |
| 4 | 1 | drop | ammonium sulfate | 2.2 (M) | |
| 5 | 1 | drop | imidazole/malate | 100 (mM) |
