1MUU
2.0 A crystal structure of GDP-mannose dehydrogenase
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97934, 0.97947, 0.94225 |
| Spacegroup name | P 43 |
| Unit cell lengths | 82.497, 82.497, 310.173 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.020 |
| R-factor | 0.20999 |
| Rwork | 0.209 |
| R-free | 0.23400 * |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.103 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.088 * | 0.338 * |
| Total number of observations | 975001 * | |
| Number of reflections | 129450 * | |
| Completeness [%] | 99.7 * | 99.4 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 323 | 8% MPD, 100 mM Na acetate, pH 4.6, VAPOR DIFFUSION, HANGING DROP, temperature 323K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | HEPES | 50 (mM) | pH7.5 |
| 2 | 1 | drop | dithiothreitol | 1.0 (mM) | |
| 3 | 1 | drop | protein | 7.5 (mg/ml) | |
| 4 | 1 | reservoir | PEG3350 | 2 (%) | |
| 5 | 1 | reservoir | magnesium sulfate | 20 (mM) | |
| 6 | 1 | reservoir | sodium acetate | 0.1 (M) | pH4.8 |
