1MUG
G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1996-10-15 |
Detector | MARRESEARCH |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 40.010, 49.050, 91.080 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 1.800 |
R-factor | 0.198 |
Rwork | 0.198 |
R-free | 0.25200 |
Structure solution method | MIR |
RMSD bond length | 0.010 |
RMSD bond angle | 1.500 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((AGROVATA) |
Phasing software | CCP4 |
Refinement software | X-PLOR (3.851) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.000 | 1.900 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.052 | 0.110 |
Number of reflections | 16296 | |
<I/σ(I)> | 8.3 | 6.2 |
Completeness [%] | 94.7 | 85.6 |
Redundancy | 3.6 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Batch method * | 4.6 | 12-20% PEG 400. 60-80MM AMMONIUM SULPHATE 0.05M SODIUM ACETATE PH 4.6 |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | 1 | protein | 4 (mg/ml) | |
2 | 1 | 1 | PEG4000 | 12-20 (%) | |
3 | 1 | 1 | ammonium sulfate | 60-80 (mM) |