1MUG
G:T/U MISMATCH-SPECIFIC DNA GLYCOSYLASE FROM E.COLI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1996-10-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.010, 49.050, 91.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25200 |
| Structure solution method | MIR |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | CCP4 |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.000 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.052 | 0.110 |
| Number of reflections | 16296 | |
| <I/σ(I)> | 8.3 | 6.2 |
| Completeness [%] | 94.7 | 85.6 |
| Redundancy | 3.6 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 4.6 | 12-20% PEG 400. 60-80MM AMMONIUM SULPHATE 0.05M SODIUM ACETATE PH 4.6 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 4 (mg/ml) | |
| 2 | 1 | 1 | PEG4000 | 12-20 (%) | |
| 3 | 1 | 1 | ammonium sulfate | 60-80 (mM) |
