1MU5
Structure of topoisomerase subunit
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-12-20 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 1.127 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.091, 110.968, 54.544 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.21579 |
| Rwork | 0.214 |
| R-free | 0.23900 * |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.313 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 * | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.058 * | 0.405 * |
| Total number of observations | 284447 * | |
| Number of reflections | 37738 | |
| <I/σ(I)> | 16.7 | 2.65 |
| Completeness [%] | 95.8 | 90.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Batch method * | 7 | 20 * | PEG 3000, Tris, calcium acetate, pH 7.0, Microbatch, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | Tris-HCl | 20 (mM) | pH7.0 |
| 2 | 1 | 1 | 100 (mM) | ||
| 3 | 1 | 1 | protein | 12 (mg/ml) | |
| 4 | 1 | 2 | PEG3000 | 20 (%) | |
| 5 | 1 | 2 | Tris-HCl | 100 (mM) | pH7.0 |
| 6 | 1 | 2 | 200 (mM) |
