1MU5

Structure of topoisomerase subunit

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	100
Detector technology	CCD
Collection date	2001-12-20
Detector	ADSC QUANTUM 4
Wavelength(s)	1.127
Spacegroup name	P 21 21 2
Unit cell lengths	94.091, 110.968, 54.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.21579
Rwork	0.214
R-free	0.23900 *
Structure solution method	MAD
RMSD bond length	0.011
RMSD bond angle	1.313 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000 *	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.058 *	0.405 *
Total number of observations	284447 *
Number of reflections	37738
<I/σ(I)>	16.7	2.65
Completeness [%]	95.8	90.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch method *	7	20 *	PEG 3000, Tris, calcium acetate, pH 7.0, Microbatch, temperature 293K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	Tris-HCl	20 (mM)	pH7.0
2	1	1		100 (mM)
3	1	1	protein	12 (mg/ml)
4	1	2	PEG3000	20 (%)
5	1	2	Tris-HCl	100 (mM)	pH7.0
6	1	2		200 (mM)