1MTY
METHANE MONOOXYGENASE HYDROXYLASE FROM METHYLOCOCCUS CAPSULATUS (BATH)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1994-12 |
| Detector | FUJI |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.700, 109.600, 330.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 5.000 - 1.700 |
| R-factor | 0.183 |
| Rwork | 0.183 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mmo |
| RMSD bond length | 0.010 |
| RMSD bond angle | 21.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 12.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.039 | 0.237 |
| Number of reflections | 188161 | |
| <I/σ(I)> | 20 | 20 |
| Completeness [%] | 77.0 | 75 |
| Redundancy | 2.5 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7 | 4 * | macroseeding * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | PEG4000 | 10 (%) | |
| 2 | 1 | reservoir | 25 (mM) | ||
| 3 | 1 | reservoir | 25 (mM) | ||
| 4 | 1 | reservoir | MOPS | 25 (mM) |
