1MTN

BOVINE ALPHA-CHYMOTRYPSIN:BPTI CRYSTALLIZATION

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	RIGAKU RAXIS II
Spacegroup name	P 61
Unit cell lengths	102.450, 102.450, 207.570
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.800
R-factor	0.18 *
R-free	0.24800 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cha
RMSD bond length	0.013
RMSD bond angle	2.480
Data reduction software	MOSFLM
Phasing software	AMoRE
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000 *
High resolution limit [Å]	2.800 *
Rmerge	0.096
Total number of observations	106612 *
Number of reflections	27327
Completeness [%]	90.3
Redundancy	3.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6	22 *	pH 6.0

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	alpha-CHT	10.0 (mg/ml)
2	1	drop	BPTI	4.0 (mg/ml)
3	1	drop	magnesium sulfate	1.0 (M)
4	1	drop	sodium acetate	0.025 (M)