1MT3
Crystal Structure of the Tricorn Interacting Factor Selenomethionine-F1
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-09-04 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.9788, 0.9795, 0.9500 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.968, 60.759, 80.678 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.285 |
| Rwork | 0.255 |
| R-free | 0.28000 |
| Structure solution method | MAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.594 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 2.000 |
| Rmerge | 0.064 |
| Number of reflections | 33131 * |
| <I/σ(I)> | 24.6 |
| Completeness [%] | 99.0 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6 | 293 | 12% PEG 6000, 100 mM MES, pH 6.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | reservoir | MES | 100 (mM) | pH6.0 |
| 3 | 1 | reservoir | Bis-Tris-HCl | 100 (mM) | pH6.0 |
| 4 | 1 | reservoir | PEG6000 | 7-12 (%) | |
| 5 | 1 | reservoir | HEPES | 100 (mM) | pH7.5 |
| 6 | 1 | reservoir | PEG8000 | 20 (%) |
