1MSY
GUAA tetraloop mutant of Sarcin/Ricin domain from E. Coli 23 S rRNA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2000-07-06 |
| Detector | CUSTOM-MADE |
| Wavelength(s) | 0.97764 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 57.974, 22.534, 58.465 |
| Unit cell angles | 90.00, 101.72, 90.00 |
Refinement procedure
| Resolution | 29.000 - 1.420 * |
| R-factor | 0.1637 |
| Rwork | 0.164 |
| R-free | 0.20700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 483d |
| RMSD bond length | 0.010 |
| RMSD bond angle | 0.028 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | SHELXL-97 |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.420 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.072 | 0.323 |
| Number of reflections | 14372 | |
| <I/σ(I)> | 21.8 | 2.3 |
| Completeness [%] | 97.9 | 80 |
| Redundancy | 6.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 8 * | 19 * | ammonium sulfate, potassium MOPS, magnesium chloride, manganese chloride, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 292K |
Crystallization Reagents
| ID | crystal ID | solution ID | reagent name | concentration | details |
| 1 | 1 | 1 | (NH4)2SO4 | ||
| 2 | 1 | 1 | potassium MOPS | ||
| 3 | 1 | 1 | MgCl2 | ||
| 4 | 1 | 1 | MnCl2 | ||
| 5 | 1 | 2 | (NH4)2SO4 | ||
| 6 | 1 | 2 | MgCl2 | ||
| 7 | 1 | 2 | MnCl2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 2.5 (mg/ml) | |
| 2 | 1 | drop | sodium EDTA | 1.0 (mM) | |
| 3 | 1 | drop | Tris | 10 (mM) | pH8.0 |
| 4 | 1 | reservoir | ammonium sulfate | 3.0-3.2 (M) | |
| 5 | 1 | reservoir | potassium MOPS | 50 (mM) | pH7.0 |
| 6 | 1 | reservoir | 10 (mM) | ||
| 7 | 1 | reservoir | 10 (mM) |
