1MSP

MAJOR SPERM PROTEIN, ALPHA ISOFORM (RECOMBINANT), PH 4.6

Experimental procedure

Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 1 2 1
Unit cell lengths	215.360, 38.480, 32.460
Unit cell angles	90.00, 93.11, 90.00

Refinement procedure

Resolution	21.000 * - 2.500
R-factor	0.195 *
R-free	0.29200 *
RMSD bond length	0.013
RMSD bond angle	21.830 *
Data reduction software	MOSFLM
Refinement software	TNT

Data quality characteristics

	Overall
Low resolution limit [Å]	21.000 *
High resolution limit [Å]	2.500 *
Rmerge	0.046
Number of reflections	8858
Completeness [%]	95.0
Redundancy	1.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	4.6		pH 4.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG8000	11 (%(w/v))
2	1	reservoir	ammonium sulfate	2 (%sat)
3	1	reservoir	ammonium acetate	50 (mM)