1MSK
METHIONINE SYNTHASE (ACTIVATION DOMAIN)
Experimental procedure
| Temperature [K] | 298 |
| Detector technology | AREA DETECTOR |
| Detector | ADSC |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 38.860, 62.360, 140.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.800 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.25700 |
| Structure solution method | MIR |
| RMSD bond length | 0.018 |
| RMSD bond angle | 24.200 * |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| High resolution limit [Å] | 1.800 |
| Rmerge | 0.053 |
| Number of reflections | 28762 |
| <I/σ(I)> | 10.1 |
| Completeness [%] | 88.0 |
| Redundancy | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 | pH 7.2 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | EDTA | 10 (mM) | |
| 4 | 1 | reservoir | PEG6000 | 30 (%) | |
| 5 | 1 | reservoir | MgAcetate | 300 (mM) | |
| 6 | 1 | reservoir | Tris-HCl | 100 (mM) | |
| 7 | 1 | reservoir | 4-5 (%) |
