1MSI
STRUCTURE OF ANTIFREEZE GLYCOPROTEIN QAE(HPLC 12)
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE X11 |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | X11 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-30 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 32.328, 38.893, 45.483 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.250 |
| R-factor | 0.198 |
| Rwork | 0.198 |
| R-free | 0.22500 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 26.330 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR (3.0) |
| Refinement software | X-PLOR (3.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.310 |
| High resolution limit [Å] | 1.250 | 1.250 |
| Rmerge | 0.049 | 0.557 |
| Total number of observations | 46873 * | |
| Number of reflections | 16529 | |
| <I/σ(I)> | 24.1 | 1.64 |
| Completeness [%] | 96.6 | 98.1 |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 20 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 3-3.5 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 25-27.5 (%sat) | |
| 3 | 1 | drop | sodium acetate | 0.05 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 50-55 (%sat) | |
| 5 | 1 | reservoir | sodium acetate | 0.1 (M) |
