1MRP

FERRIC-BINDING PROTEIN FROM HAEMOPHILUS INFLUENZAE

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	292
Detector technology	IMAGE PLATE
Collection date	1996-12
Detector	MARRESEARCH
Spacegroup name	P 21 21 2
Unit cell lengths	132.194, 52.449, 41.255
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
R-factor	0.175 *
Rwork	0.174
R-free	0.24000
Structure solution method	MIR
RMSD bond length	0.017 *
RMSD bond angle	2.280 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASES
Refinement software	TNT (5E)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.660
High resolution limit [Å]	1.600	1.600
Rmerge	0.072 *	0.282 *
Number of reflections	36442
<I/σ(I)>	27.3	2.1
Completeness [%]	93.0	54
Redundancy	3.1	1.54

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	6.6		20% PEG 4000, 400 MM IMIDAZOLE/MALATE PH 6.6, 200 MM NACL

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG4000	20 (%)
2	1	reservoir		200 (mM)
3	1	reservoir	imidazole/malate	400 (mM)