1MRO
METHYL-COENZYME M REDUCTASE
Experimental procedure
| Source type | SYNCHROTRON |
| Source details | EMBL/DESY, HAMBURG BEAMLINE BW7B |
| Synchrotron site | EMBL/DESY, HAMBURG |
| Beamline | BW7B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-04 |
| Detector | MAR scanner 345 mm plate |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.720, 116.883, 122.582 |
| Unit cell angles | 90.00, 92.02, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.160 |
| R-factor | 0.197 |
| Rwork | 0.197 |
| R-free | 0.20700 |
| Structure solution method | MIR |
| RMSD bond length | 0.013 |
| RMSD bond angle | 24.000 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.3) |
| Refinement software | CNS (0.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 1.230 |
| High resolution limit [Å] | 1.160 | 1.160 |
| Rmerge | 0.066 | 0.330 |
| Number of reflections | 735767 | |
| <I/σ(I)> | 18 | 3 |
| Completeness [%] | 93.0 | 89 |
| Redundancy | 3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 25% PEG 400 0.2 M NACL 20 MG/ML FINAL PROTEIN CONC 0.2 M MGCL 01.M HEPES PH 7.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | PEG400 | 25 (%) |
