1MR7

Crystal Structure of Streptogramin A Acetyltransferase

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2000-11-10
Detector	MARRESEARCH
Wavelength(s)	0.97947, 0.97939, 0.96863
Spacegroup name	F 2 2 2
Unit cell lengths	181.393, 183.001, 184.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 * - 1.800
R-factor	0.17792
Rwork	0.176
R-free	0.20700 *
Structure solution method	MAD
RMSD bond length	0.012
RMSD bond angle	1.240 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.840
High resolution limit [Å]	1.800	1.800
Rmerge	0.053	0.438
Total number of observations	1293725 *
Number of reflections	140296
<I/σ(I)>	21.3	3.27
Completeness [%]	97.2 *	91.3 *
Redundancy	3.37

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K
1	VAPOR DIFFUSION, HANGING DROP	7.5 *	290	Sodium Formate 2.7M, VAPOR DIFFUSION, HANGING DROP, temperature 290K

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	protein	0.15-0.25 (mM)
2	1	reservoir	Tris-HCl	1 (mM)	pH7.5