1MQT

Swine Vesicular Disease Virus coat protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-4
Synchrotron site	ESRF
Beamline	ID14-4
Temperature [K]	100
Detector technology	CCD
Collection date	2002-06-19
Detector	ADSC QUANTUM 4
Wavelength(s)	0.939
Spacegroup name	I 2 2 2
Unit cell lengths	318.350, 349.960, 371.720
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 - 3.000 *
Rwork	0.257
R-free	0.27900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cov
RMSD bond length	0.012 *
RMSD bond angle	1.900 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	DM
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	3.500
High resolution limit [Å]	3.000 *	3.000 *
Rmerge	0.100 *	0.191 *
Total number of observations	459326 *
Number of reflections	288614 *
<I/σ(I)>	4.6
Completeness [%]	70.0 *	51 *
Redundancy	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.2 *	293	Jimenez-Clavero, M.A., (2003) Acta Cryst., D59, 541. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	virus solution	5-10 (mg/ml)
2	1	reservoir	ammonium sulfate	1.2 (M)
3	1	reservoir	sodium potassium phosphate	0.1 (M)