1MQK
Crystal structure of the unliganded Fv-fragment of the anti-cytochrome C oxidase antibody 7E2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MPG/DESY, HAMBURG BEAMLINE BW6 |
| Synchrotron site | MPG/DESY, HAMBURG |
| Beamline | BW6 |
| Temperature [K] | 277 |
| Detector technology | IMAGE PLATE |
| Collection date | 1993-06-12 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.436, 56.045, 99.846 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.280 |
| R-factor | 0.136 |
| Rwork | 0.136 |
| R-free | 0.19600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qle |
| RMSD bond length | 0.021 |
| RMSD bond angle | 2.280 * |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((AGROVATA) |
| Phasing software | X-PLOR |
| Refinement software | SHELXL |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.500 | 1.310 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.040 * | 0.218 |
| Total number of observations | 178789 * | |
| Number of reflections | 64251 | |
| <I/σ(I)> | 28.6 | 5.2 |
| Completeness [%] | 85.1 | 63.4 |
| Redundancy | 2.8 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 6 | 277 | sodium phosphate, pH 6.0, MICRODIALYSIS, temperature 277K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 12 (mg/ml) | |
| 2 | 1 | 1 | sodium phosphate | 100 (mM) | pH6.0 |
